Fig. 2
Viral loads in collected tissues and fecal samples from mice at five different time points after infection with four virus strains. Mice were inoculated with 106 EID50/mL of the JS/10, KR/07, MV/12 and CA/09 strains. In each virus group, the brain (a), heart (b), liver (c), lung (d), spleen (e), kidney (f), intestine (g) and feces (h) were collected from the mice to determine the viral loads using real-time PCR at 1, 4, 7, 11 and 14 days post-challenge. *, P < 0.05, or **, P < 0.01, indicates significantly different virus titers compared between group JS/10 and KR/07. #, P < 0.05, or ##, P < 0.01, indicates significantly different virus titers compared between group MV/12 and KR/07. For viral loads in different organs mentioned above, the results are expressed as log10 (viral RNA copies)/g. The horizontal line means the detection limit of this assay (158 copies of RNA per g)