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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Virulent duck enteritis virus infected DEF cells generate a unique pattern of viral microRNAs and a novel set of host microRNAs

Fig. 5

Luciferase reporter assay for the interaction between dev-miR-D8-3p and US1 gene. a The seed sequence of dev-miR-D8-3p and its target site in 3’UTR of the US1 mRNA are shown in red, seven nucleotides were mutated in 3’UTR of the US1 mRNA (underlined). b Activity of the luciferase gene linked to the 3’UTR of the US1 mRNA. The wild-type pmirGLO-WT-US1 (WT-US1) and mutant pmirGLO-MU-US1 (MU-US1) were respectively transfected into COS7 cells with the dev-miR-D8-3p (miR-D8-3p) mimic or the negative control (miR-NC). Luciferase activities were measured after 36 h. The data were presented as the means and the standard deviations (SDs) of separate transfections (n = 3). Statistical significance was analyzed by one-way analysis of variance (ANOVA). The significant differences (p < 0.05) are indicated as single star

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BMC Veterinary Research

ISSN: 1746-6148

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