Table 1 The ratio of unbound free DNA residue was determined by calculating the image intensity of free DNA fragments on an electrophoretic agarose gel after a VP1-DNA binding assay combining increasing amounts of recombinant GST-VP1 protein with certain DNA molecule conformations
From: Characterization of the DNA binding activity of structural protein VP1 from chicken anaemia virus
| Â | Concentrations of GST-VP1 protein (ng) | ||||
|---|---|---|---|---|---|
0 | 100 | 300 | 500 | 700 | |
Open circular dsDNA (Form I) | 100% | 100% | 100% | 59.6% | 11% |
Supercoiled dsDNA (Form II) | 100% | 100% | 82.6% | 0% | 0% |
Linear dsDNA | 100% | 93.8% | 84.7% | 36.5% | 0% |
Linear ssDNA (−) | 100% | 92.9% | 53.3% | 0% | 0% |
M13 phage DNA | 100% | 90% | 46.5% | 0% | 0% |