Fig. 4
From: Safety and efficacy of the mesenchymal stem cell in feline eosinophilic keratitis treatment
Assessment of adipogenic (a-f) and osteogenic (g-k) differentiation. Control (a-c) and adipo-induced (d-f) cultures at 7, 14 and 21 days. Positive Oil Red O staining confirmed the presence of lipid droplets only in adipogenic-induced cells. Bars, 200 μm. Inserts represent higher magnification of the specific stain. Histochemical localization of ALP (g-h) and Alizarin Red S (i-j) staining at day 21. Osteoinduced cells formed numerous nodules highly positive for ALP staining. Control cells remained Alizarin Red S negative by day 21 whereas red calcium nodules clearly appeared on the osteoinduced cultures. k Quantitative measurement of ALP activity at 7, 14 and 21 days. Osteoinduced cells exhibited significantly higher levels of ALP activity (p < 0.001) compared with the controls. Values represent the means ± SD, n = 3. Asterisk (*) indicates a statistically significant difference (p < 0.001) for the same condition at different time points, whereas (●) represents p < 0.001 between two groups at the same time period. Bars, 200 μm