Fig. 3
From: Safety and efficacy of the mesenchymal stem cell in feline eosinophilic keratitis treatment
Cell morphology, proliferation and representative FACS analysis of fAd-MSCs for several mesenchymal and hematopoietic markers. a In primary cultures, a large number of adherent cells with fibroblastic morphology and colony-forming units were observed two days after initial seeding. b On secondary cultures, fAd-MSCs appeared as spindle-shaped cells that grown in a monolayer. c Representative curve obtained with MTS cell proliferation assay at passage 2. Cells started proliferating immediately after being plated, initiating the logarithmic growth phase and reaching its plateau phase around 21 days. d The immunophenotype profiles revealed a homogeneous cell population, characterized by the strong positive expression of CD29, CD44, CD73, CD90 and major histocompatibility class I (MHC-I), and lack expression of CD34, CD45 and MHC-II. As well, a minor population of STRO-1-expressing cells was observed. Bars, 200 μm