Table 1 Details on the calculations used in the ELISA analyses, the cut-off values employed and the correlation between the ELISA results and antibody levels

BRSV

450 nm

PP = Sample_COD /P_COD X100

PP ≥ 10

Positive

BPI3V

450 nm

PP ≥ 10

Positive

BCoV

450 nm

PP ≥ 10

Positive

BHV-1 gE

650 nm

S/N ratio = Sample_OD/N₂

S/N ≤ 0.6

Negative

BHV-4

450 nm

S/P ratio = (Sample_OD – N₁)/(P₁-N₁)

S/P ≥ 0.3

Positive

BVD

450 nm

S/P ratio = (Sample_OD – N₂)/(P₂-N₂)

S/P ≥ 0.3

Positive

H. somni

450 nm

S/P ratio = (Sample_OD – N₃)/(P₃-N₃)

S/P > 0.6

Positive