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Table 1 Subject and procedures for collection of olfactory mucosa

From: Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

Genus (species)

Gender & population

Strain & Origin

Age

Anaesthesia & analgesia procedure

Collection method

Mouse (Mus musculus)

Male, 6a

C57Bl/6NCrl, Charles River Laboratories

3 months

Euthanasia; sodium pentobarbital (Ceva Santé Animale, 200 mg/kg, i.p.)

Muzzle dissection

Rat (Rattus norvegicus)

Male, 6a

Fischer 344, Charles River Laboratories

6 weeks

Sodium pentobarbital (Nembutal, 60 mg/kg, i.p.) and Buprenorphine (0.02 mg/kg i.m.)

Nasal bone trepanation

Rabbit (Oryctolagus cuniculus)

Male, 3

Female,3

Breed, IRSEA

11 (± 1.5) months

Medetomidine (Domitor®, 0.4 mg/kg i.m.), Ketamine 100 (Imalgene®, 30 mg/kg i.m.) and Buprenorphine (0.02 mg/kg i.m.)

Nasal cavity exploration

Dog (Canis lupus)

Male, 4 Female, 2

Multiple, pet dogsb

15 (± 1) years

Zoletil (Tiletamine and Zolazepam,Virbac, 5 mg/kg i.v.) and Boutorfanole (Dolorex®, 0.1 mg/kg i.v.)

Nasal cavity exploration

Horse (Equus caballus)

Male, 4

Multiple, client-ownedb

18 (± 3) years

Détomidine (Domosedan®, 10–20 μg/kg i.v.)

Nasal cavity exploration

Sheep (Ovis aries)

Female, 3

Merinos, IRSEA

7 (± 4) years

Xylazine (Rompun® 2%, 0.04 mg/kg i.v.)

Nasal cavity exploration

Gray mouse Lemur (Microcebus murinus)

Male, 1 Female, 1

CNRS Primatology centre

7 years

Diazepam (Valium, 20 mg/kg i.m.) and Ketamine (Imalgene, 100 mg/kg i.p.)

Nasal bone trepanation

Macaque (Macaca fascicularis)

Male, 1

CNRS Primatology centre

13 years

Zoletil (Tiletamine and Zolazepam,Virbac, 5 mg/kg i.m.); then isoflurane (Belamont)

Nasal cavity exploration

  1. amucosa from different individuals were pooled
  2. bclient-owned animals from veterinary clinics biopsied with owner consent