Influence of silver nanoparticles on growth and health of broiler chickens after infection with Campylobacter jejuni

Table 2 Genes and primers used in the study

RNA target gene	Primer sequences(5′–3′)	PCR Product		Gene bank Assess. No
RNA target gene	Primer sequences(5′–3′)	Annealing Temperature	Amplicon (bp)	Gene bank Assess. No
GAPDH				NM_204305.1
Forward	TGCTGCCCAGAACATCAT	61 °C	199
Reverse	ATCAGCAGCAGCCTTCAC
ACTB				NM_205518.1
Forward	GTCCACCTTCCAGCAGATGT	60 °C	169
Reverse	ATAAAGCCATGCCAATCTCG
NF-kB				M86930.1
Forward	TTGCTGCTGGAGTTGATGTC	60 °C	167
Reverse	TTGCTGCTGGAGTTGATGTC
TNF-α				NM_204267.1
Forward	TTCAGATGAGTTGCCCTTCC	59 °C	150
Reverse	TCAGAGCATCAACGCAAAAG

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, ACTB Actin beta, NF-kB nuclear factor kappa B, and TNF-α Tumor necrosis factor alpha

ISSN: 1746-6148