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Table 1 Identification of 2D–spots which appeared differentially regulated under stress (T15) in comparison to basal levels (T0) from pooled saliva samples of pigs subjected to snaring restraint

From: Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva

Sample preparation

Spot number

Mean T0

Mean T15

P value

Protein identificationa

SP1

5

0.23

1.05

0.0001

Prolactin inducible protein

20

0.53

1.07

0.0011

Salivary lipocalinc

76

1.68

0.99

0.0011

Prolactin inducible proteinc

49

0.39

0.28

0.0050

Adenosine deaminase

48

0.29

0.21

0.0064

Adenosine deaminase

30

0.13

0.20

0.0089

Light chain of immunoglobulinsb

29

0.14

0.44

0.0148

Light chain of immunoglobulinsb

75

0.04

0.61

0.016

No identification

24

2.85

1.45

0.0218

Salivary lipocalin

47

0.24

0.15

0.0219

Adenosine deaminase

17

0.18

0.52

0.0243

Salivary lipocalin

99

0.22

0.15

0.0295

Light chain of immunoglobulinsb

27

0.20

0.50

0.0353

Salivary lipocalin

37

0.31

0.58

0.0385

Carbonic anhydrase VI

92

2.97

1.30

0.0405

Salivary lipocalin

12

1.62

1.02

0.0421

Prolactin inducible proteinc

28

0.14

0.26

0.0481

Light chain of immunoglobulinsb

SP2

34

0.21

0.86

0.0006

No identification

129

0.13

0.13

0.0007

No identification

36

1.80

1.15

0.0032

Prolactin inducible proteinc

28

0.25

0.16

0.0046

No identification

0

0.63

0.42

0.0053

Protein S100-A12

77

0.53

0.83

0.0068

Carbonic anhydrase VI

35

2.59

1.9

0.0104

Lipocalin 1

38

1.10

0.45

0.0139

Prolactin inducible protein

56

1.42

0.86

0.0161

Salivary lipocalin

2

0.42

0.64

0.0379

Protein S100-A8

114

0.31

0.18

0.0410

Immunoglobulin M

33

1.40

0.62

0.043

No identification

SP3

10

0.77

1.79

0.0002

Double headed protease inhibitor submandibular gland

6

1.76

3.55

0.0006

Protein S100-A12

136

0.09

0

0.0020

No identification

60

1.13

0.68

0.0092

Protein S100-A9

5

1.10

2.00

0.0101

Hemoglobinc

16

2.10

1.23

0.0104

Protein S100-A9

33

0.33

0.15

0.0170

Adenosine deaminase

78

0.85

0.62

0.0260

Light chain of immunoglobulinsb

150

0.90

0.25

0.0318

No identification

34

0.34

0.71

0.0326

No identification

17

0.59

0.36

0.0463

No identification

  1. SP1: sample preparation 1, untreated pooled saliva. SP2: sample preparation 2, glycoprotein enrichment fraction of pooled saliva samples. SP3: sample preparation 3, dynamic range compression of pooled saliva samples
  2. aIdentifications obtained by comparison to previous MS data [16, 17]. bIdentifications obtained by comparison to previous Western blot data [17]. c MS identifications performed in the present study (Table 2)
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BMC Veterinary Research

ISSN: 1746-6148

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