Fig. 5

DgRIG- I and chMDA5 overexpression enhances IBV-induced IFN-β transcription. a DF1 cells were transfected with the indicated plasmid for 24 h and then infected with IBV for 24 h. The extracted RNA was used to measure the expression of IFN-β. The expression of IFN-β in the test group was compared to the mock control group that was transfected with the control vector and infected with IBV. The growth properties of IBV in chTLR3-, chMDA5- and dgRIG-I-overexpressed cell supernatants titrated onto CEK cells are expressed as TCID₅₀/ml (b) or by RT-qPCR to determine IBV genome load (c). Experiments were performed in triplicate, and data are representative of three independent experiments (* P ≤ 0.05; ** P ≤ 0.01)