Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow

BMC Veterinary Research

Table 1 Primers sequences used for qPCR

Gene symbol¹	GenBank accession	Primer sequence (5′ to 3′)²	Melting temperature	Product size
SLC2A1	NM_174602.2	F: GACACTTGCCTTCTTTGCCA	58.97	164 bp
SLC2A1	NM_174602.2	R: AACCTAATGGAGCCTGACCC	59.08	164 bp
SLC2A4	NM_174604.1	F: TCATTCTTGGACGGTTCTTC	55.40	159 bp
SLC2A4	NM_174604.1	R: CTAGCACCTGGGCGATTA	56.08	159 bp
SLC2A8	NM_201528.1	F: CGGCTCAGAACCTGTGGAT	59.40	165 bp
		R: GGAGGATGCCTGTGACTACC	59.53
SLC2A12	NM_001011683.2	F: ATTGTCATCGGCATTCTT	51.80	165 bp
SLC2A12	NM_001011683.2	R: ATGTCCCTTCATCACCAG	53.43	165 bp
SLC35A2	NM_176640.2	F: GTGGTCCAGAATGCTTCCCTC	60.68	163 bp
		R: CCAGGTGCTTCACGTTACCC	60.95
SLC35B1	NM_181338.2	F: GACCTGCTCCATCATCACCAC	60.75	126 bp
		R: AGACCGAGACCCAAGAACACC	61.71
AKT1	NM_173986.2	F: TCATGCAGCACCGATTCTT	57.44	159 bp
AKT1	NM_173986.2	R: CTTGGTCAGGTGGCGTAAT	57.45	159 bp
HK2	XM_015473383.1	F: AAGATGCTGCCCACCTACG	59.78	123 bp
HK2	XM_015473383.1	R: TCGCTTCCCATTCCTCACA	58.63	123 bp
LALBA	NM_174378.2	F: AGACTTGAAGGGCTACGGA	57.62	177 bp
LALBA	NM_174378.2	R: TAGTTGCTTGAGTGAGGGTT	56.38	177 bp
B4GALT-I	NM_177512.2	F: TGCCCTGAGGAGTCCCC	59.92	151 bp
B4GALT-I	NM_177512.2	R: GGCCACCTTGTGAGGAGAG	59.70	151 bp
ACTB	NM_173979.3	F: AGGACCTCTACGCCAACACG	61.87	249 bp
ACTB	NM_173979.3	R: TTTGCGGTGGACGATGGAG	60.38	249 bp

¹SLC2A1 = glucose transporter 1(GLUT1); SLC2A4 = glucose transporter 4 (GLUT4); SLC2A8 = glucose transporter 8 (GLUT8); SLC2A12 = glucose transporter 12 (GLUT12); SLC35A2 = solute carrier family 35 member A2; SLC35B1 = solute carrier family 35 member B1; AKT1 = PKB alpha; HK2 = hexokinase 2; LALBA = α-lactalbumin (α-LA); B4GALT1 = β-1,4-galactosyltransferase 1 (β4GalT-I); ACTB = β-ACTIN
²F: forward; R: reverse

ISSN: 1746-6148