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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream

Fig. 1

Dynamic range and sensitivity of the real-time PCR assay for LCDV detection. a Standard curve obtained using dilutions of plasmid DNA ranging from 106 to 2 copies per reaction. Linear regression was performed on mean data from four separate runs. The logarithm to base 10 (log) of the plasmid copy number versus the cycle threshold (Ct) value is represented. b Standard curve showing a linear relationship between the log of the amount of infective virus (expressed in TCID50) per reaction and their corresponding Ct values

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