Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study

BMC Veterinary Research

Table 1 Immunoreactivity scoring of VIM, α-sma, Type I collagen, Type III collagen, MMP-2, MMP-9, MMP14, TIMP-2 in 25 equine sarcoids and 5 normal skin

Samples^a	Location	VIM^b	Α-SMA^b	Type I collagen^b	Type III collagen^b	MMP-2^b	MMP-9^b	MMP14^b	TIMP-2^b
T1	Abdomen	++	-	+	+	+	+	+/−	++
T2	Limbs	++	-	+	+	++	+	+/−	++
T3	Neck	++	-	+	+	+	+	+/−	++
T4	Abdomen	++	-	+	+	+	+/−	+/−	++
T5	Paragenit region	++	-	+	+	++	+	+	++
T6	Pectoral region	++	-	+	+	+	+/−	+	++
T7	Paragenit region	++	-	+	+	+	+/−	+	++
T8	Neck	++	-	+	+	++	+	+	++
T9	Neck	++	-	+	+	+	+/−	+/−	++
T10	Pectoral region	++	-	++	+	+	+	+/−	++
T11	Paragenit region	++	-	++	+	++	+	+/−	++
T12	Limbs	++	-	++	+	++	+	+/−	++
T13	Neck	++	-	++	+	+	+/−	+	++
T14	Abdomen	++	-	++	+	+	+/−	+	++
T15	Limbs	++	-	++	+	++	+	+/−	++
T16	Pectoral region	++	-	++	+	+	+/−	+/−	++
T17	Pectoral region	++	-	++	+	+	+/−	+/−	++
T18	Limbs	++	-	++	+	++	+	+	++
T19	Abdomen	++	-	++	+	+	+/−	+	+
T20	Pectoral region	++	-	++	+	+	+/−	+/−	+
T21	Limbs	++	++	++	+	++	+	+/−	+
T22	Neck	++	++	++	+	++	+	+/−	+
T23	Abdomen	++	++	++	+	+	+/−	+	+
T24	Paragenit region	++	++	++	+	+	+/−	+	+
T25	Pectoral region	++	++	++	+	+	+/−	+	+
N1	Limbs	n.a.	n.a.	+/−	+/−	+	+/−	-	-
N2	Abdomen	n.a.	n.a.	+/−	+/−	+	+/−	+/−	+/−
N3	Pectoral region	n.a.	n.a.	+/−	+/−	+	+/−	-	+/−
N4	Neck	n.a.	n.a.	+/−	+/−	+	+/−	+/−	-
N5	Limbs	n.a.	n.a.	+/−	+/−	+	+/−	+/−	+/−

^a T tumour sample, N normal skin sample, ^b- negative staining; +/− weak immunolabelling; + moderate immunolabelling; ++ extensive and strong immunolabelling; n.a. not assessed

ISSN: 1746-6148