Fig. 3

Rescue of BoHV-4-ΔIE1 impaired replication in trans. a Schematic diagram (not on scale) of BoHV-4IE1 gene containing exons (A, B, C and D) and introns (dashed lines), along with its reverse transcribed cDNA, amplified by PCR with a couple of primers containing restriction sites and to allow its subcloning into the bicistronic vector. b Replication kinetics of BoHV-4ΔIE1 (red line) and BoHV-4 (blue line) on BEK/IE1 cells