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Fig. 2 | BMC Veterinary Research

Fig. 2

From: A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB

Fig. 2

SDS-PAGE analysis of recombinant bacteria of BL21 (DE3)(pCold-rGP5) (a), BL21(DE3)(pGEX-6p-1-rGP5) (b) and BL21 (DE3)(pCold-rGP5-fljB) (c). (a) Lanes: M, molecular weight markers; 1, product of BL21(DE3)(pCold) induced by IPTG; 2, Lysate supernatant of bacteria bearing His-rGP5 induced by IPTG; 3, Inclusion bodies of bacteria bearing His-rGP5 induced by IPTG; 4, product of bacteria bearing His-rGP5 not induced. (b) Lanes: M, molecular weight markers; 1, product of BL21(DE3)(pGEX-6p-1) induced by IPTG; 2, Lysate supernatant of bacteria bearing GST-rGP5 induced by IPTG; 3, Inclusion bodies of bacteria bearing GST-rGP5 induced by IPTG. (c) Lanes: M, molecular weight markers; 1, product of BL21(DE3)(pCold) induced by IPTG; 2, product of bacteria bearing rGP5-FljB not induced; 3, Lysate supernatant of bacteria bearing rGP5-FljB induced by IPTG; 4, Inclusion bodies of bacteria bearing rGP5-FljB induced by IPTG

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BMC Veterinary Research

ISSN: 1746-6148

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