Figure 1

Description and timeline of immunization protocol. Piglets (n = 5/group) were gavaged with ovalbumin (OVA) (0.05 mg or 0.5 mg) + 50 μg unmethylated oligonucleotides containing CG oligodeoxynucleotides (CpG 2395) as a soluble vaccine as well as with 0.5 mg OVA + 50 μg CpG 2395 within a 0.5 mg PCEP polyphosphazene microparticles (MP). Gavages took place within six hours of birth. The negative control (saline) group and the i.p. control group were gavaged with saline. With the exception of the saline control group, the remaining groups were i.p. immunized at four weeks of age with 10 mg OVA in Incomplete Freunds’ Adjuvant (IFA). At eight weeks of age, piglets were euthanized and lung lavages were harvested. Blood was obtained on day three, day seven and then weekly (grey arrows). At time of death, blood was drawn for PBMC isolation.