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Table 5 Characteristics of primers used for qPCR analysis

From: Effects of dietary polyphenol-rich plant products from grape or hop on pro-inflammatory gene expression in the intestine, nutrient digestibility and faecal microbiota of weaned pigs

Gene1

Forward primer (from 5'to 3')Reverse primer (from 5'to 3')

PCR product size (bp)

NCBI GenBank

Reference genes

ATP5G1

CAGTCACCTTGAGCCGGGCGA

94

NM_001025218.2

TAGCGCCCCGGTGGTTTGC

ACTB

GACATCCGCAAGGACCTCTA

205

XM_003124280.3

ACATCTGCTGGAAGGTGGAC

GAPDH

AGGGGCTCTCCAGAACATCATCC

446

NM_001206359.1

TCGCGTGCTCTTGCTGGGGTTGG

GPI

CACGAGCACCGCTCTGACCT

365

NM_214330.1

CCACTCCGGACACGCTTGCA

RPS9

GTCGCAAGACTTATGTGACC

327

XM_005664825.1

AGCTTAAAGACCTGGGTCTG

SDHA

CTACGCCCCCGTCGCAAAGG

380

DQ402993

AGTTTGCCCCCAGGCGGTTG

NF-κB and nutrient transporter target genes

CCL2

GGTCCTTGCCCAGCCAGATGC

170

NM_214214.1

CTGCACAGATCTCCTTGCCCGC

ICAM1

CGGTGGCAGCCGTGGCTATC

208

NM_213816.1

TTGATGCAGCCCCGCTCGTC

IL1B

GTTCTCTGAGAAATGGGAGC

143

NM_214055.1

CTGGTCATCATCACAGAAGG

IL8

ACTTCCAAACTGGCTGTTGC

120

NM_213867.1

GGAATGCGTATTTATGCACTGG

SLC2A2

GCTGGATGGGGAAGCCAAAGCA

355

NM_001097417.1

AGAGCGTCGCCCTGCCTTCT

SLC2A5

CTGACACTGGTGCTTGCTTT

156

EU012359.2

TTCGCTCATGTATTCCCCGA

SLC5A1

GTGGCGGACAGTAGTGAACA

89

NM_001164021.1

AGAAGGCAGGATTTCAGGCA

SLC15A1

CAGACTTCGACCACAACGGA

99

NM_214347.1

TTATCCCGCCAGTACCCAGA

TNF

CATGAGCACTGAGAGCATGA

170

NM_214022.1

CGATAACTTCGAAGTGCAGT

Bacterial group

Bifidobacterium spp.

TCGCGTC(A/T)GGTGTGAAAG

243

 

CCACATCCAGC(A/G)TCCAC

Clostridium Cluster XIVa

AAATGACGGTACCTGACTAA

485

 

CTTTGAGTTTCATTCTTGCGAA

Lactobacillus spp.

AGCAGTAGGGAATCTTCCA

341

 

CACCGCTACACATGGAG

Streptococcus spp.

AGAGTTTGATCCTCCGTCAG

144

 

GTTAGCCGTCCCTTTCTGG

  1. 1ATP5G1 = ATP synthase lipid-binding protein; ACTB = actin beta; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; GPI = glucose-6-phosphate isomerase; RPS9 = ribosomal protein; SDHA = succinate dehydrogenase complex, subunit A; CCL2 = chemokine (C-C motif) ligand 2; SLC2A2 = solute carrier family 2 (facilitated glucose transporter) 2; SLC2A5 = solute carrier family 2 (facilitated glucose transporter) 5; SLC5A1 = sodium-glucose transporter 1; SLC15A1 = solute carrier family 15 (oligopeptide transporter), member 1; ICAM1 = intercellular adhesion molecule; IL = interleukin; TNF = tumor necrosis factor.
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BMC Veterinary Research

ISSN: 1746-6148

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