Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR

Table 3 Estimation of the proviral copy number (per 1 mL peripheral blood ^a ) in each lymphocyte subpopulation using BLV-CoCoMo-qPCR

Cattle	Proviral load (Copies/10⁵cells)	Proviral copy number/ml of blood in each of the lymphocyte subpopulations
		CD5⁺IgM⁺	CD5^-IgM⁺	CD4⁺	CD8⁺
1) BLV-infected cattle ( proviral load > 100 copies/10⁵ cells)
N733	1,614	268,914	7,809	11,980	8,811
N788	18,094	360,816	99,429	133,782	111,587
O10	10,689	450,514	36,951	88,382	81,626
2) BLV-infected cattle (proviral load < 100 copies/10⁵ cells)
N818	8	148	328	91	60

^aThe proviral load in each lymphocyte subpopulation (expressed as the copy number per 10⁵ PBMCs) was estimated by BLV-CoCoMo-qPCR [19] and then calculated in terms of copies per ml.

ISSN: 1746-6148