An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis

Table 2 Primers used for MLVA in this study

Marker	Primer sequence^a		Primer concentration
	Forward	Reverse
fnVNTR-1	V-CAGCTAGAAGCTTATTCGCCTCTT	CAGGGTAATGCCTTAACGCATAT	0.2 μM
fnVNTR-2	F-GAGTATTCCCTGCACCTACAATGAT	TGTCATGCCTTTTTCTCTAGAGGAT	0.2 μM
fnVNTR-3	N-CAAACTCTTTCACAAGAGGAAGCAT	TCTGAACTCTGCTCTTTTCCCTCTA	0.2 μM
fnVNTR-4	F-TTGTGCAAACACATCGATAGGAGAT	AACTGCATCATCAGCATCTCTTCTA	0.2 μM
fnVNTR-4^b	GTGCTTTTGCCTGTACCACCTT	GCATCATTTGATACACCGTCCA	NA
fnVNTR-5	N-CAATCACTCATCAACCACTAGCCAT	TGCTGACTAATGGCTGACTGTAGTT	0.3 μM
fnVNTR-5^b	CTCATCAACCACTAGCCATCACAT	AAAGCCTGGGCTATCTAAATGCT	NA

^aFluorescent dyes are abbreviated as follows: F, FAM (6-carboxyfluorescein); N, NED; V, VIC. ^bsequencing primers; NA, not applicable.

ISSN: 1746-6148