Figure 4

Phosphorylation of CREB by ritanserin is evident in COS but not in CnOb. a: Immunoblots of CREB and phosphorylated CREB in CnOb. Immunoblot of CnOb confirming the absence of endogenous CREB expression. No treatments resulted in an induction of CREB. b: Immunoblots of CREB and phosphorylated CREB in COS. CREB phosphorylation increases at high concentrations of serotonin and intermediate to high concentrations of ritanserin, or with a combination of the two drugs.