Reaction 1 | Reaction 2 | Reaction 3 | |||||||
---|---|---|---|---|---|---|---|---|---|
Theoretical copy number | Average Ct | Average calculated copy number | CV % | Average Ct | Average calculated copy number | CV % | Average Ct | Average calculated copy number | CV % |
MVV assay | |||||||||
10 6 | 20 | 853389 | 8.67% | 20.04 | 1008108 | 23.29% | 19.83 | 1007657 | 7.96% |
10 5 | 23.29 | 105939 | 9.25% | 23.40 | 102693 | 9.62% | 23.32 | 97177 | 9.14% |
10 4 | 26.69 | 12190 | 3.14% | 26.66 | 11365 | 1.43% | 26.64 | 10483 | 7.21% |
10 3 | 30.29 | 1248 | 6.60% | 30.07 | 1136 | 5.75% | 30.16 | 990 | 7.14% |
10 2 | 34.65 | 83 | 37.84% | 34.01 | 79 | 1.35% | 33.59 | 103 | 34.05% |
CAEV assay | |||||||||
10 5 | 21.22 | 87689 | 38.76 | 20.96 | 94514 | 31.38 | 21.71 | 92044 | 30.09 |
10 4 | 23.55 | 17265 | 18.81 | 24.41 | 11326 | 17.53 | 24.91 | 11230 | 16.01 |
10 3 | 27.33 | 1463 | 13.65 | 28.16 | 1160 | 20.64 | 27.94 | 1193 | 3.18 |
10 2 | 31.15 | 66 | 39.76 | 32.58 | 79 | 17.85 | 33.06 | 55 | 1.84 |
10 | 34.4 | 11 | 46.69 | 35.73 | 13 | 58.26 | 35.56 | 11 | 2.3 |