Table 7 RT-PCR primer sets for canine RTKs
RTK | Primers | Product size (bp) |
|---|---|---|
c-Kit | 232 F: 5’ -- GAG AAC ACA CAC AAC GAA TG – 3’ 414R: 5’ -- GCA GCG GAC CAG CGT ATC ATT G – 3’ | 185 |
PDGFRα | 1917 F: 5’ – GCT CTC ATG TCG GAA CTG AAG – 3’ 2152R: 5’ – GTG TGC TGT CAT CAG CAG G – 3’ | 237 |
PDGFRβ | 2235 F: 5’ – GAC GAG TCA GTG GAT TAC GTG – 3’ 2562R: 5’ – GTC TCT CAT GAT GTC ACG AGC CAG – 3’ | 329 |
c-Ret | 206 F: 5’ – GCC ACT GTG ATG CTG TAG AGA GCA G – 3’ 259R: 5’ – GTG CGG CAG AGC TCA TCA CAC AGT GG – 3’ | 210 |
VEGFR2 | 1537 F: 5’ – GTA AGT ACC CTT GTT ATC CAA GCA GCC – 3’ 1728R: 5’ – CGT AGT TCT GTC TGC AGT GCA CCA C – 3’ | 195 |
GAPDH | 526 F: 5’ – GTC CAT GCC ATC ACT GCC ACC CAG – 3’ 718R: 5’ – CTG ATA CAT TGG GGG TGG GGA CAC – 3’ | 196 |