Increased hypoxia-inducible factor 1α expression in lung cells of horses with recurrent airway obstruction

BMC Veterinary Research

Table 3 Primer sequences used for RT-qPCR validation procedure

Genes	Equine accession number	Primers sequence		Product size (bp)
		5′--------------------------------- > 3′
Targets
Hif1-α	LOC100061166	Forward	ctcaaatgcaagaacctgctc	108
Hif1-α	LOC100061166	Reverse	ttccataccatcttttgtcactg	108
VEGF-α	NM_001081821	Forward	tggcagaaggagagcataaaa	123
VEGF-α	NM_001081821	Reverse	actcgatctcatcggggtact	123
IL-8	NM_001083951	Forward	aatgagagcgattgagagtgg	127
IL-8	NM_001083951	Reverse	caaaaacgcctgcacaataat	127
TNF-α	EU438779	Forward	agcctcttctccttcctcctt	123
TNF-α	EU438779	Reverse	cagagggttgattgactggaa	123
Housekeeping gene
ACTB	NM_001081838.1	Forward	ggacctgacggactacctc	81
ACTB		Reverse	cacgcacgatttccctctc	81
GAPDH	NM_001163856	Forward	atctgacctgccgcctggag	70
GAPDH	NM_001163856	Reverse	cgatgcctgcttcaccaccttc	70
HPRT	XM_001490189.2	Forward	aattatggacaggactgaacgg	121
HPRT	XM_001490189.2	Reverse	ataatccagcaggtcagcaaag	121
RPL32	None	Forward	gggagcaataagaaaacgaagc	113
RPL32	None	Reverse	cttggaggagacattgtgagc	113
SDHA	XM_001490889.3	Forward	gaggaatggtctggaatactg	91
SDHA	XM_001490889.3	Reverse	gcctctgctccataaatcg	91

ISSN: 1746-6148