Figure 2

Comparison of BLV detection by BLV-CoCoMo-qPCR, nested PCR, and serological tests. (A) BLV proviral load, as evaluated by BLV-CoCoMo-qPCR, in whole blood from 370 cattle that were either positive (+) or negative (−) for BLV LTR sequences, as determined by nested PCR, and serological tests such as the passive hemagglutination reaction (PHA), agar gel Immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA). Bars show the median BLV proviral load values. The actual number of cattle that were positive by BLV-CoCoMo-qPCR alone per number of cattle that were either positive (+) or negative (−) for BLV infection as determined by each test is indicated at the upper of each block. (B) Nested PCR positive rates for different proviral copy numbers per 10⁵ cells (copy number of 0-10⁵), as evaluated by BLV-CoCoMo-qPCR. The positive rate was 1.3 % when the proviral load was 0.