Molecular typing of Coxiella burnetii from animal and environmental matrices during Q fever epidemics in the Netherlands

Table 2 Results of fragment analyses and sequencing of PCR products obtained from samples of commercial dairy farms

Locus	Fragment	PCR product length (bp)		Number of tandem repeats
Locus	Fragment	Fragment analysis	Sequencing	Fragment analysis¹	Sequencing
Ms27	1	285	282	2.4	2.0
	2	290	288	3.3	3.0
	3	295	294	4.2	4.0
Ms28	1	189	190	2.8	3.0
	2	201	202	4.8	5.0
	3	207	208	5.8	6.0
	4	213	214	6.8	7.0
Ms34	1	107	112	1.2	2.0
	2	113	118	2.2	3.0
	3	124	130	4.1	5.0
	4	136	142	6.1	7.0
	5	143	148	7.1	8.0
	6	148	153	8.1	9.0
Ms20b	1	252	258	4.4	4.5
	2	269	273	4.9	5.0
	3	349	354	7.3	7.5
Ms24	1	165	163	9.3	9.0
	2	179	177	11.3	11.0
	3	192	191	13.1	13.0
	4	287	289	26.7	27.0
Ms31	1	130	136	2.1	3.0
	2	145	150	4.3	5.0

¹The numbers of tandem repeats from fragment sizes were calculated by dividing the sizes of the measured PCR products minus primer binding sites and flanking regions by the corresponding repeat length.

ISSN: 1746-6148