Figure 2

Fluorescent microscopy showing the cytopathic effects (CPE) of OFTu cells infected with OV-IA82Δ113 and OV-IA82Δ116 by using limited dilution (A) and plaque purification (B). A. Limited dilution showed OFTu cells infected with OV-IA82Δ113 and OV-IA82Δ116 after 24 h pi. The strong fluorescent signal was observed in OFTu cells infected with both OV-IA82Δ113 and OV-IA82Δ116. (b) and (e) show the same fields as in (a) and (d) by bright-field microscopy. (c) and (f), overlay of (a) and (b) or (d) and (e). Cells were visualized in a Leica SP2 microscope in 63 × magnification. Bar, 10 μm. B. Plaque purification showed the plaques with strong fluorescent signal in OFTu cells infected with OV-IA82Δ113 (a, b, c) and OV-IA82Δ116 (d, e, f) at 36 h pi during the second round of plaque purification. (b) and (e) show the same fields as in (a) and (d) by bright-field microscopy. (c) and (f), overlay of (a) and (b) or (d) and (e). Cells were visualized in a Leica DMI4000B inverted microscope in 10× magnification. Bar, 20 μm.