Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR

BMC Veterinary Research

Table 1 Comparison of primer specificity using reference strains of Mycobacteria commonly isolated from clinical samples.

	Primers
	Conventional PCR			Real-time PCR
Mycobacterium species	IS6110^a	IS41/IS43^b	IS6110_T	IS6110_T
M. bovis	+	+	+	+
M. terrae	+	+
M. goodii	+	+	+
M. fortuitum		+
M. kansasii
M. avium subsp. Paratuberculosis
M. wolinskyi	+	+	+	+
M. simiae
M. peregrinum	+
M. intracellulare
M. chelonae	+
M. avium subsp avium
M. smegmatis

ISSN: 1746-6148