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Table 2 Treatment effects on haematological variables pre and post-transport in control and transported (RT and ULT) animals (n = 20 animals/treatment)

From: The effect of road and sea transport on inflammatory, adrenocortical, metabolic and behavioural responses of weanling heifers

Variable

d 0 pre-transport

d 2 Arrival in French lairage

D 3 post-12 h lairage

d 4 arrival in Spain

d 6 post-transport

d 8 post-transport

d 10 post-transport

d 34 post-transport

White blood cell (WBC) number (1×103 μL)

Control

9.20 ± 0.57ax

8.20 ± 0.41abx

8.60 ± 0.54abx

7.90 ± 0.75abx

8.90 ± 0.75abx

7.81 ± 0.49abx

7.40 ± 0.44bx

9.66 ± 0.47bx

RT

10.10 ± 0.57ax

20.90 ± 2.30by

15.40 ± 1.99by

23.10 ± 1.43by

15.00 ± 1.43by

12.60 ± 1.01by

18.70 ± 1.74by

13.10 ± 1.22by

ULT

9.00 ± 0.59ax

16.70 ± 16.0by

14.0 ± 1.16by

17.80 ± 1.31by

15.60 ± 1.31by

14.10 ± 1.45by

16.10 ± 1.07by

11.14 ± 0.95by

Neutrophil percentage (%)

       

Control

36.25 ± 2.70abx

32.95 ± 2.705bx

38.22 ± 3.10abx

33.20 ± 3.00bx

35.95 ± 2.85bx

38.10 ± 2.11abx

42.10 ± 2.42ax

32.44 ± 3.30bx

RT

38.00 ± 2.72abx

37.16 ± 2.76abx

39.20 ± 2.97abx

43.95 ± 3.01bx

37.16 ± 2.92abx

42.67 ± 2.21bx

33.79 ± 2.50axy

40.68 ± 3.10abx

ULT

37.30 ± 2.70abx

34.35 ± 2.70abx

37.78 ± 3.10abx

38.84 ± 3.00abx

34.55 ± 2.85abx

37.53 ± 2.25abx

31.47 ± 2.48aby

36.06 ± 3.23abx

Lymphocyte percentage (%)

       

Control

62.35 ± 2.86abx

64.26 ± 2.74abx

60.11 ± 3.02bx

65.85 ± 2.92abx

62.85 ± 2.87abx

60.40 ± 2.05abx

57.30 ± 2.47ax

64.88 ± 14.14abx

RT

59.80 ± 2.90abx

58.63 ± 2.70abx

59.15 ± 2.92abx

53.89 ± 3.00bx

61.37 ± 2.14abx

54.94 ± 2.53bx

65.68 ± 3.10axy

58.42 ± 15.62abx

ULT

59.15 ± 2.80abx

62.05 ± 2.70abx

59.89 ± 3.01abx

59.00 ± 2.96abx

63.25 ± 2.86abx

60.82 ± 2.20abx

68.32 ± 2.53aby

62.82 ± 12.51abx

Neutrophil:Lymphocyte ratio

       

Control

0.81 ± 0.13abx

0.70 ± 0.08abx

0.73 ± 0.10abx

1.00 ± 0.13bx

0.68 ± 0.09abx

0.82 ± 0.06abx

0.59 ± 0.08ax

0.89 ± 0.13abx

RT

0.70 ± 0.13abx

0.61 ± 0.08abx

0.79 ± 0.10abx

0.81 ± 0.13bxy

0.64 ± 0.08abx

0.65 ± 0.06by

0.49 ± 0.07axy

0.64 ± 0.13abx

ULT

0.69 ± 0.13abx

0.58 ± 0.78abx

0.70 ± 0.10abx

0.54 ± 0.13aby

0.63 ± 0.08abx

0.67 ± 0.06abxy

0.80 ± 0.07aby

0.57 ± 0.14abx

Haematocrit (HCT) (%)

       

Control

39.49 ± 0.57axy

39.09 ± 0.41abx

38.32 ± 0.54bx

36.71 ± 0.66bx

36.51 ± 0.75bx

36.54 ± 0.49bx

35.39 ± 0.44bx

33.69 ± 0.47bx

RT

39.18 ± 0.67ax

34.41 ± 0.74by

32.17 ± 1.25by

32.26 ± 0.56by

31.36 ± 0.65by

29.75 ± 0.68by

29.78 ± 0.63by

28.09 ± 0.66by

ULT

40.23 ± 0.59ay

33.63 ± 0.90by

32.06 ± 1.16by

32.98 ± 1.69by

32.01 ± 1.31by

30.97 ± 1.45by

30.74 ± 1.07by

28.97 ± 0.95by

  1. The values are expressed as mean ± s.e. Control = not transported; RT (transported animals that remained on the transporter at the French lairage for 12 h) and ULT animals transported (transported animals that were unloaded at the French lairage for a 12 h rest period) at a stocking density of 0.93 m2 per animal;a,bWithin a row, means not having a common superscript differ significantly (P < 0.05);x,ywithin a column at each sampling time point (day (d)), treatment means differ by P < 0.05. Data were analysed using SAS/STAT (9.1 (SAS Inst. Inc., Cary, NC, USA). The differences between means were tested using the Tukey-Kramer test for multiple comparisons.