Skip to main content

Table 3 Polymorphic microsatellite markers for canine DCM candidate genes1

From: Canine candidate genes for dilated cardiomyopathy: annotation of and polymorphic markers for 14 genes

Gene

Repeat

Primers (5'-3')

Forward;

Reverse

Ta (°C)

Detected alleles

Informativeness2

Origin (bp ... of contig AAEX 010...)

Distance to gene3

    

bp

#

PIC

#chr

  

ACTC

15CA

actccgaagaaggaagtcaac4

gttcccatctatgagggctat

57.0

234–238

2

0.17

10

bp 37,720/..13479

69.2 kb downstr. Stop

 

20CA

ggaacaaggtgctgttagacc5

cacattccaccgagtaggc7

59.0

338–356

5

0.42

24

bp 5,945/..13478

intragenic (intron 4)

CAV1

13CA

ccacagagctagaaagctacg4

tgttgcaaacaccctatgat

54.5

240–242

2

0.28

24

bp 39,315/...48546

8.2 kb downstr. Stop

CSRP3

15CA

catgtcctgcaagttaatggt4

ggatttctattctgggtttcc

53.0

237–245

3

0.41

24

bp 38,367/..17412

2.8 kb upstr. Start

LMNA

16CA

gggtggtagatgagcatttc6

gaagagaacaagtgggcaag

54.5

204–212

4

0.36

12

bp 5,128/..12734

9.3 kb downstr. Stop

 

18GAAA

ggaagatgagactgttagaatgc5

caggccatgattacttttcc7

57.0

321–344

6

0.67

24

bp 26,754/..12735

22.6 kb downstr. Stop

MYH7

21CA

gatatcctgggattaaagactgg5

ctattttgccctcttcatgg7

58.0

351–363

4

0.37

24

bp 1,418/..41098

36.6 kb downstr. Stop

TNNI3

20CAa

tcaaacagggaaacctgaac6

gattattcagctcccagaacc7

57.0

297–301

3

0.38

24

bp 597/..53929

119.3 kb upstr. Start

 

20CAb

ttccagttgattgtttctctgc5 gcggtttagcactgcattc7

59.0

302–306

2

0.08

24

bp 13,248/..53916

110.2 kb downstr. Stop

 

17GA

tccaacctcagggtactgg5 catgccatggagctatgc7

59.0

304–312

3

0.37

24

bp 48,910/..53930

179.8 kb upstr. Start

TPM1

19CA8

actgtgtccagagtgcagcta4

gattgctagactggc

60.0

467–483

4

0.67

12

bp 88,113/..08742

6.5 kb downstr. Stop

VCL

15GAAA9

caatttcttttccaatcacattag 10

gccattttgcattctcttcaa

54.0

150–170

6

0.69

24

bp 12,680/..16406

88.6 kb downstr. Stop

  1. 1 Microsatellites for DES and SGCD were demonstrated in, respectively, [28] and [30]; 2 The informativeness of each SNP was described by its polymorphism information content (PIC), based on the number of genotyped chromosomes (#chr) listed; 3 Based on genomic build 1.1; 4 F-primer fluorescently labelled with 6FAM; 5 Three-primer protocol used; 6 F-primer fluorescently labelled with HEX; 7 extra tail on R-primer: GTGTCTT---- (5'-3') to promote addition of an Adenosine residue at the 3'-end of the complementary DNA strand; 8 Microsatellite demonstrated in [31]; 9 Personal communication P.Stabej; 10 F-primer fluorescently labelled with TET.
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us