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Table 2 Reproducibility of PPRV N gene based real time RT-PCR assay

From: A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus

a. Intra-assay variation

DNA copies/μl

 

Ct values

 

Mean Ct

S.D

5.11 × 107

10.71

10.77

10.89

10.79

0.09

5.11 × 106

14.52

14.81

14.75

14.69

0.15

5.11 × 105

18.70

18.91

18.22

18.61

0.35

5.11 × 104

21.77

21.75

21.58

21.70

0.10

5.11 × 103

25.56

24.45

24.74

24.92

0.58

5.11 × 102

27.07

27.27

26.89

27.08

0.19

5.11 × 101

30.57

30.74

30.57

30.63

0.10

5.11 × 10°

32.52

33.26

32.59

32.79

0.41

b. Inter-assay variation

DNA copies/μl

 

Ct values

 

Mean Ct

S.D.

Assay 1

Assay 2

Assay 3

5.11 × 107

10.75

10.79

11.24

10.93

0.27

5.11 × 106

14.84

14.70

16.17

15.24

0.81

5.11 × 105

18.92

18.61

20.33

19.29

0.92

5.11 × 104

22.07

21.7

23.14

22.30

0.75

5.11 × 103

24.67

24.91

24.57

24.72

0.17

5.11 × 102

27.42

27.08

27.61

27.37

0.27

5.11 × 101

31.13

30.63

32.83

31.53

1.15

5.11 × 10°

33.12

32.79

33.74

33.22

0.48

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BMC Veterinary Research

ISSN: 1746-6148

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