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Table 1 Detection of anti-HEV antibody in swine sera by ELISA-ORF2, ELISA-kit, and Western blotting assays

From: Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein

ELISA -ORF2 (%)*

ELISA-kit (%)

Western blotting (%)

S/CO ELISA-ORF2

p

S/CO ELISA-kit

p

Observed frequency

Estimated frequency

Probability of infection**

+

+

+

5.40

<0.01

5.15

<0.05

75

71

1

+

+

-

3.79

 

4.61

 

23

23

0.89

+

-

+

4.69

<0.05

1.51

<0.05

4

5

0.98

+

-

-

2.30

 

1.31

 

2

3

0.56

-

+

+

0.99

<0.05

2.10

<0.05

1

3

0.93

-

+

-

0.73

 

3.69

 

3

3

0.33

-

-

+

0.81

<0.05

1.80

<0.05

1

0

0.61

-

-

-

0.63

 

1.75

 

2

2

0.05

104 (93.7)

102 (91.9)

81 (73.0)

    

111

  
  1. *positive/total tested.
  2. **estimated by the model.