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Table 2 Primer sequences for amplification of ORF3 and ORF5 genes from PRRSV strain LV

From: Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus

Name

Sequence (5′ → 3′)

P1

CGCGGATCCGCTCATCAGTGTGCACGCTTCCAT

P2

CCCAAGCTTTCGTGATGTACTGGGGAGTACCG

P3

CGGGATCCGGCAACGGCGACAGCTC

P4

CCCTCGAGGGCCTCCCATTGCTCAG

P5

CGCGGATCCAGATGTTCTCACAAATTGGGGCGTT

P6

CCCAAGCTTGGCCTCCCATTGCTCAGCCG

P7

CGCGGATCCGGAGGCGGAGGCTCCGGAGGAGGAGGCTCCGGAGGCGGAGGGTCTAGATGTTCTCACAAATTGGGGCGTT

  1. Primers P1/P2 were for full-length ORF3; P3/P4 were for ORF5 lacking the signal peptide; primers P5/P6 were for full-length ORF5, primer P7 was for the full-length sequence of ORF5; the italics part encodes the G4S flexible Linker.
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BMC Veterinary Research

ISSN: 1746-6148

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