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Fig. 7 | BMC Veterinary Research

Fig. 7

From: Use of immune repertoire sequencing to resolve discordant microscopic and immunochemical findings in a case of T cell-rich large B cell lymphoma in a young dog

Fig. 7

Immune repertoire analysis, liver and ascites, dog. a Bar plots depicting the lymphocyte antigen receptor gene diversity of lymphocyte infiltrates in ascites (top row) and liver (second row) of the immunoglobulin heavy chain locus (IGH) (left column), T cell receptor beta (TRB) (middle column) and T cell receptor gamma (TRG) (right column) loci as well as a lymph node from a dog without lymphoma (pc control). There is an unequivocal clonal IGH result for the liver sample; all other samples show polyclonal results for all loci consistent with reactive T cells. X-axis: junctional length in amino acids; y-axis: clonotype abundance in reads. Colored bar slices represent the 100 most abundant clonotypes per sample, grey bar slices are less abundant clonotypes. Double bars per junctional length indicate replicate samples. b Abundance of the 5 most frequent clonotypes, liver and ascites, dog. The most abundant IGH clonotype in the liver comprises greater than 80% of all reads and the line plot drops sharply after the most frequent clone; in contrast, all TRB and TRG clonotypes are below 3% forming a near horizontal line. Bottom row: polyclonal control (pcc). X-axis clonotype rank; y-axis clonotype abundance in percent of total reads per sample. c Heat map illustrating the overlap of the 20 most frequent IGH (left column), TRB (middle column) and TRG (right column) clonotypes in the liver and ascites of a dog with hepatic T cell-rich B cell lymphoma as well as a lymph node from a dog without lymphoma (pc control) and a negative control (nt control). Each column represents a sample and each row represents a clone. The color intensity of each tile corresponds to the read abundance supporting each clone; abundance is given as the mean of two replicates. The IGH repertoire is dominated by a single (neoplastic) clone with no discernible repertoire overlap between liver and ascites. In contrast, the TRB and TRG clonotypes are found in the liver and the ascites but not in the polyclonal control or the negative control

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