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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Use of immune repertoire sequencing to resolve discordant microscopic and immunochemical findings in a case of T cell-rich large B cell lymphoma in a young dog

Fig. 4

Immunocytochemistry (ICC) of abdominal effusion fluid collected at postmortem, dog. a Three small lymphocytes had strong cytoplasmic immunopositivity for CD3, while intermediate and large lymphocytes had faint cytoplasmic immunopositivity for CD3. The background contained numerous erythrocytes. ICC for CD3, 600x magnification. b Granulocytes were negative for CD3. ICC for CD3, 1000x magnification. c Rare small lymphocytes had cytoplasmic immunopositivity for CD20. The majority of the lymphocytes in the fluid were immunonegative for CD20. ICC for CD20, 600x magnification. d CD20 immunopositivity is apparent only in small lymphocytes. ICC for CD20, 1000x magnification

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