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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Use of immune repertoire sequencing to resolve discordant microscopic and immunochemical findings in a case of T cell-rich large B cell lymphoma in a young dog

Fig. 3

Flow cytometric analysis of abdominal effusion cells. Gating on cells with high forward scatter (FSC) and high side scatter (SSC) (top row) identifies cells that are nearly all positive for CD5, MHC II and CD45, and 56% that are positive for CD4. Note faint CD3 positivity on a few cells, and absence of CD8 and CD21 detection. Gating on cell populations with high and low FSC (bottom row) shows that almost all cells are MHC II and CD45 positive, confirming that they are lymphocytes. A proportion are also CD4 (22%) and CD5 (41%) positive. Detection of CD3 is faint, and CD8 or CD21 positive cells are rare

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