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Table 3 Blood biochemical parameters in ponies class A, Felin ponies and Polish Koniks compared with so far published reference intervals for various types of horses (according to 1Rossdale & Partners, 2011 [5], 2Hinchcliff & Geor, 2013 [1], 3Latson 2005 [9], Henderson 2013 [10])

From: Variations in haematological and biochemical parameters in healthy ponies

Parametera

Ponies class A (n = 73)

Felin ponies

(n = 28)

Polish Koniks

(n = 41)

General linear model p-value for the effect of

Reference intervals

groups

sex

ageb

group & sex interaction

“hot-blooded” horses

“cold-blooded” horses

American Miniature Horses

ALP [U/l]

185 ± 81

(92–756)AB

149 ± 55

(72–281)A

211 ± 46 (126–358)B

0.036

0.327

0.104

0.424

   

AST [U/l]

384 ± 83 (228–648)A

321 ± 50 (233–423)B

395 ± 65 (221–536)A

0.007

0.153

0.770

0.941

234 ± 163 (Clydesdales)2 172 ± 28 (Shire)2

189 ± 332

234 ± 163 (Clydesdales)2 172 ± 28 (Shire)2

GGTP [U/l]

18, 15–21

(8–53)A

20, 18–25 (14–48)A

55, 45–82 (20–198)B

< 0.001

0.059

0.055

< 0.001

24.2 ± 6.0 (Shire)2

11 ± 4.42

24.2 ± 6.0 (Shire)2

CPK [U/l]

473, 381–587 (171–1233)A

361, 277–408 (192–609)B

599, 459–769 (306–1137)A

0.001

0.037

0.855

0.059

56 ± 22 (Clydesdales)2

58 ± 12 (Shire)2

273 ± 1362

56 ± 22 (Clydesdales)2

58 ± 12 (Shire)2

TP [g/l]

66.8 ± 4.9 (56–75)

63.9 ± 5.6 (50–75)

66.1 ± 3.7 (57–75)

0.275

0.268

0.182

0.250

72 ± 6 (Clydesdales and Shire)2

66 ± 62

72 ± 6 (Clydesdales and Shire)2

ALB [g/l]

35.6 ± 3.2 (26–41)A

37.8 ± 2.6 (34–46)B

36.1 ± 1.9 (32–41)A

0.003

0.116

0.635

0.684

36 ± 5 (Clydesdales)2

28 ± 3 (Shire)2

 

36 ± 5 (Clydesdales)2

28 ± 3 (Shire)2

Urea [mmol/L]

7.0 ± 1.4

(3.8–10.2)

7.0 ± 1.1

(5.1–9.0)

8.2 ± 1.5

(3.8–13.0)

0.812

0.034

0.776

0.019

5.2 ± 1.14 (Clydesdales)2

8.5 ± 1.42

5.2 ± 1.14 (Clydesdales)2

Creatinine [μmol/L]

95.8 ± 16.9 (46.8–130.0)

98.8 ± 16.3 (61.9–137.0)

77.6 ± 17.1 (47.7–116.7)

0.357

0.004

0.983

0.051

151 ± 26.5 (Clydesdales)2

88.4 ± 17.72

151 ± 26.5 (Clydesdales)2

TBIL [μmol/L]

16.9 ± 5.3 (5.3–32.3)A

20.6 ± 6.4 (11.1–37.6)A

12.3 ± 4.6 (4.4–28.7)B

0.001

< 0.001

0.216

0.015

   

TG [mmol/L]

0.30 ± 0.15 (0.1–0.8)A

0.42 ± 0.20 (0.2–0.9)B

0.33 ± 0.12 (0.2–0.6)AB

0.029

0.504

0.002

−0.27 (−0.44, −0.10)

0.038

   

Glucose [mmol/L]

5.4 ± 0.9

(3.9–8.4)A

5.1 ± 1.1

(3.6–9.1)A

3.8 ± 0.9

(2.2–6.3)B

< 0.001

0.270

0.809

0.138

4.21 ± 1.1 (Clydesdales)2

5.1 ± 0.72

4.21 ± 1.1 (Clydesdales)2

Lactate [mmol/L]

1.1, 0.9–1.4 (0.1–2.7)A

0.1, 0.1–0.1 (0.1–0.9)B

0.1, 0.1–0.1 (0.1–2.6)B

< 0.001

0.428

0.157

0.534

234 ± 163 (Clydesdales)2 172 ± 28 (Shire)2

189 ± 332

234 ± 163 (Clydesdales)2 172 ± 28 (Shire)2

  1. ALP Alkaline phosphatase, AST Aspartate aminotransferase, GGTP Gamma-glutamyl transpeptidase, CPK Creatine kinase, TP Total protein concentration, ALB Albumin concentration, TBIL Total bilirubin, TG Triglycerides
  2. a presented as arithmetic mean ± SD and range in parenthesis except for CPK, GGTP, and lactate which were non-normally distributed and presented as the median, interquartile range (IQR) and range in parenthesis
  3. b standardized regression coefficient with 95% confidence interval (CI 95%) presented if significant
  4. A-B groups marked by different capital letters differ significantly in between-group pairwise comparisons at α = 0.05,