Fig. 4

Mycobacterium avium paratuberculosis MAP vaccination induced cellular and humoral response in ewes during the transition period. Ewes were vaccinated at d 14 a.p, blood samples were collected before the vaccination (0) and 24 h following the vaccination (1) to investigate the cellular compositions (a, b, c, d, e, f, g, h and i), whereas serum samples were collected at d 7 and d 21 postvaccination to assess the MAP Abs response (j). Two-way ANOVA test and Sidak multiple comparisons test were used to detect differences between control (n = 9) and Mg (n = 10) groups as well as between time points. Significant time-dependent differences (P ≤ 0.05) are indicated by different letters. Significant differences between the two groups are indicated as* (P < 0.05) and ** (P < 0.01). Mean ± SEM