Fig. 6

Effect of AJSAF on cytokine secretion from splenocytes in the immunized mice. Splenocytes were incubated with Con A (48 h) or H9–Ag (72 h), and the supernatants were collected for detecting IL-2, IFN-γ, and IL-10 levels using ELISA kits. The values are presented as means ± SD (n = 6). Significant differences with IH9V alone group were designated as ^*P < 0.05, ^†P < 0.01, and ^‡P < 0.001; those with CH9V group as ^#P < 0.01 and ^¶P < 0.001