Fig. 2From: Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponinsTime-response relationship of H9–Ag-specific IgG and its isotypes in the IH9V-immunized mice induced by AJSAF. Mice were s.c. immunized with 0.2 ml of IH9V (107 TCID50/0.1 ml) alone or containing AJSAF, or 0.2 ml of CH9V (107 TCID50/0.1 ml). Sera were collected at designated time after a single dose of vaccination for measuring H9–Ag-specific IgG, IgG1, IgG2a, and IgG2b antibody titers. The values are presented as means ± SD (n = 5). Significant differences with IH9V alone group were designated as *P < 0.05, †P < 0.01, and ‡P < 0.001; those with CH9V group as §P < 0.05, #P < 0.01, and ¶P < 0.001Back to article page