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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Fig. 3

The effects of transportation-induced transport stress on omasum of goats. a Hematoxylin and eosin stained-omasum sections from control and transportation-induced transport stress goats. Scale bar 100 μm. b Immunohistochemical staining showing the expression of HSP27, HSP70 and HSP90 in omasum of goats from control group and transported groups. NC, negative control. Scale bar 50 μm. c Western blot of the HSP27, HSP70 and HSP90 proteins in the omasum of 2 h and 6 h transportation-treated goats. β-Actin was used as loading control. d Quantitative analysis of western blot using Image Pro Plus 6.0. e Real-time PCR analysis of the HSP27, HSP70 and HSP90 mRNA levels in the omasum of 2 h and 6 h transportation-treated goats. f ELISA analysis of the HSP27, HSP70 and HSP90 concentrations in the omasum of 2 h and 6 h transportation-treated goats. Data are presented the means ± SD; *p < 0.05

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