Table 3 Primers used for qPCR for determination of endometrial LPAR1–4 mRNA level at different stages of endometrosis
Gene name | Primer sequence (5′ – 3′) | Amplicon length (bp) | Accession no. GenBank |
|---|---|---|---|
SDHA | GAGGAATGGTCTGGAATACTG | 91 | DQ402987.1 |
GCCTCTGCTCCATAAATCG | |||
LPAR1 | TTCAACTCTGCCATGAACC | 85 | XM_014735958 |
GCTGGCAGCAGAGGATCT | |||
LPAR2 | CTCAGCCGCTCCTACCTG | 71 | XM_023625558 |
TAGACAGCCACCATGAGCAG | |||
LPAR3 | TCGCAGCAGTGATCAAAAAC | 122 | XM_001917815 |
TGGGCCAGTGTTAAACATCA | |||
LPAR4 | GGCAATGCTACTGCCAATAA | 104 | XM_001501421 |
TTGGTTATCAGACCCAGGATG |