Fig. 3From: IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogsImmunoblotting for crude cod extracts. In the left column, the molecular standard is shown. Lanes 1 and 5 were stained by Coomassie brilliant blue R250. Lanes 2 and 3 depict immunoblotting in the sera samples of two atopic dogs (lane 2, dog no. 34; lane 3, dog no. 128); lane 4 represents the serum of a healthy dog used as negative control. The arrow and arrowhead next to lane 1 indicate the bands analyzed by liquid chromatography-tandem mass spectrometry, which corresponded to the band detected by immunoblotting (immunoblotting in lanes 2 and 3)Back to article page