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Table 11 Primers used for qPCR analysis

From: Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler challenged with E. coli O78

PrimerSequenceReference
TNF-αfor: 5′-GAGCTGTGGGGAGAACAAAAGGA-3′
Rev.: 5′-TTGGCCCTTGAAGAGGACCTG-3′
Tabatabaei et al. [49]
NF-ϰBfor: 5′-CAAGGCAGCAAATAGACGAG-3′
Rev.: 5′-GTTGAGAGTTAGCAGTGAGGCA-3’
GAPDHFor- 5’CCTCTCTGGCAAAGTCCAAG3′
Rev- 5’CAACATCAAATGGGCAGATG3’
  1. TNF-α: Tumor necrosis factor-alpha, NF-ϰB: Nuclear factor-kappa B, GAPDH: Glyceraldehyde-3 phosphate dehydrogenase
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