Changes in ruminal and reticular pH and bacterial communities in Holstein cattle fed a high-grain diet

BMC Veterinary Research

Table 4 Copy number of mcrA and 16S rRNA genes identified form qRT-PCR

Items	HAY Period		CON Period		SEM	P value
Items	Rumen (n = 4)	Reticulum (n = 4)	Rumen (n = 4)	Reticulum (n = 4)	SEM	Period (P)	Location (L)	P x L
Total methanogens	5.78	5.34	4.73	3.97	0.23	0.004	0.018	0.583
Fibrobacter succinogenes	5.88	4.41	3.45	2.25	0.48	< 0.001	0.119	0.600
Megasphaera elsdenii	2.97	2.89	3.76	5.99	0.64	0.180	0.741	0.230
Streptococcus bovis	7.00	6.74	4.89	7.62	0.55	0.465	0.279	0.279
Ruminococcus albus	6.13	4.65	4.76	4.45	0.37	0.068	0.123	0.147
Ruminococcus flavefaciens	7.60^a	6.03^b	5.87^a	6.42^c	0.45	0.270	0.582	0.019
Selenomonas ruminantium	7.20	6.65	6.21	6.11	0.24	0.041	0.135	0.481

ISSN: 1746-6148