Fig. 1

Daily changes in 24-h mean ruminal and reticular pH during the HAY and CON periods. Ruminal and reticular pH were measured simultaneously during the experimental period. Cattle were fed a high-forage diet for 7 days (HAY period) and then a high-grain diet for 7 days (CON period). A SARA challenge was performed as a high-forage period followed by a high-grain period. ^* denotes difference (P < 0.05) between the rumen and reticulum at the same day point. ^# denotes difference (P < 0.05) between the last day of the HAY period (day 7) and other day points at the same location. The values shown are means ± SEM