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Table 3 Primer sequences of candidate genes (reference and target genes) in the RT-qPCR assay

From: Long-term feeding with high plant protein based diets in gilthead seabream (Sparus aurata, L.) leads to changes in the inflammatory and immune related gene expression at intestinal level

Gene

Abbreviation

GeneBank ID

Primer Forward

Primer Reverse

Lenght

Reference

REFERENCE GENES

 Elongation Factor 1α

ef1α

AF184170

CTGTCAAGGAAATCCGTCGT

TGACCTGAGCGTTGAAGTTG

87

[16, 20]

 Glyceraldehide 3-phosphate dehydrogenase

gapdh

DQ641630

CCAACGTGTCAGTGGTTGAC

AGCCTTGACGACCTTCTTGA

80

[17]

 Ribosomal Protein S18

rps18

AM490061

AGGGTGTTGGCAGACGTTAC

CGCTCAACCTCCTCATCAGT

97

[17]

 β-Actin

βact

X89920

TCTGTCTGGATCGGAGGCTC

AAGCATTTGCGGTGGACG

113

[19]

TARGET GENES

 Interleukin 1β

il1β

AJ277166

GCGACCTACCTGCCACCTACACC

TCGTCCACCGCCTCCAGATGC

131

[17]

 Interleukin 6

il6

AM749958

AGGCAGGAGTTTGAAGCTGA

ATGCTGAAGTTGGTGGAAGG

101

[16]

 Interleukin 8

il8

JX976619

GCCACTCTGAAGAGGACAGG

TTTGGTTGTCTTTGGTCGAA

164

[16, 20]

 Tumor Necrosis Factor α

tnfα

AJ413189

CAGGCGTCGTTCAGAGTCTC

GAGATCCTGTGGCTGAGAGG

83

[17]

 Cyclooxygenase 2

cox2

AM296029

GAGTACTGGAAGCCGAGCAC

GATATCACTGCCGCCTGAGT

192

[16, 20]

 Caspase 1

casp1

AM490060

ACGAGGTGGTGAAACACACA

GTCCGTCTCTTCGAGTTTCG

92

[16]

 Intestinal Mucin

imuc

JQ277712

GTGTGACCTCTTCCGTTA

GCAATGACAGCAATGACA

102

[19]

 Mucin 2

muc2

JQ277710

ACGCTTCAGCAATCGCACCAT

CCACAACCACACTCCTCCACAT

90

[19]

 Mucin 2-like

muc2l

JQ277711

GTGTGTGGCTGTGTTCCTTGCTTTGT

GCGAACCAGTCTGGCTTGGACATCA

67

[19]

 Mucin 13

muc13

JQ277713

TTCAAACCCGTGTGGTCCAG

GCACAAGCAGACATAGTTCGGATAT

67

[19]

 Mucin 19

muc19

JQ277715

TGCTTGCTGATGACACAT

TTCACATAGGTCCAGATATTGA

128

[19]

 Immunoglobulin M

igm

JQ811851

TCAGCGTCCTTCAGTGTTTATGATGCC

CAGCGTCGTCGTCAACAAGCCAAGC

131

[18]

 Occludin

ocl

JK692876

GTGCGCTCAGTACCAGCAG

TGAGGCTCCACCACACAGTA

81

[16, 20]

 Tubulin

tub

AY326430

AAGATGTGAACTCCGCCATC

CTGGTAGTTGATGCCCACCT

98

[16]

 α-Amylase

αamy

AF316854

TGGTGGGACAATCAGAGTCA

GTCCAGGTTCCAGTCGTCAT

85

[16, 20]

 Alkaline phosphatase

alp

AY266359

TTACTGGGCCTGTTTGAACC

GATCTTGATGGCCACTTCCAC

102

[16, 20]

 Trypsin

tryp

AF316852

GGTCTGCATCTTCACCGACT

AAAGGCAGCAGAGTGATGGT

85

[20]

 Peptide transporter 1

pept1

GU733710

TTGAACATAACGTCGGGTGA

AATTTTGCATTTCCCTGTGG

92

[16]