Fig. 2

Gross and histological changes and viral detection in neonatal piglets affected with TGEV in case 2. Piglets presented with stool-stained perineum (a). Intestinal loops have thin walled, distended by gas and contain abundant yellow-watery diarrhea with 5–6 pH (b). Multiple sections of small intestine showed villous atrophy, blunting and fusion (c) with occasional cytoplasmic vacuoles (d). The presence of TGE mRNA confirmed in positive ISH-RNA enterocytes through multiple section of jejunum and ileum (e). f shows intracytoplasmic particles characterized by finely granular electrodense nucleoids with electron lucent centers compatible coronavirus