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Table 5 Predicted HPAIV detection percentage at different days post infection of a broiler flock by testing three (3) dead-bird pooled swab samples of sizes 5, 6 or 11 using lateral flow immunoassay FluDETECT with varying test sensitivities (se) together with 0.3% daily mortality trigger*

From: Quantifying the effect of swab pool size on the detection of influenza A viruses in broiler chickens and its implications for surveillance

Days post flock infection

1

2

3

4

5

6

7

Flock infection prevalence (%) #

0.0

0.1

0.4

2.7

13.0

32.5

48.8

1a

 3 pools of 5: se = 71%

6

27

64

88

96

99

100

 3 pools of 6: se = 71%

5

29

65

88

96

99

100

 3 pools of 11: se = 71%

6

31

69

89

97

99

100

2b

 3 pools of 6: se = 68.1%

5

27

65

88

97

99

100

 3 pools of 11: se = 57.9%

5

26

63

85

95

99

100

  1. *All results i.e. infection prevalence and detected proportions are presented as percentages
  2. #The mean simulated flock size was estimated as 24,111 birds
  3. aScenario 1: assuming that LFI test sensitivity for HPAIV is the same across pool sizes
  4. bScenario 2: assuming that LFI test sensitivity for HPAIV is different across pool sizes