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Table 3 Summary results on positive proportions and virus titer present in the pooled-swab samples; unless stated otherwise, the results are for samples that are considered RT-PCR positive i.e., those with CT ≤ 35

From: Quantifying the effect of swab pool size on the detection of influenza A viruses in broiler chickens and its implications for surveillance

Subtype

Pool size

Number RT-PCR positive/Total (% a)

Mean titer for PCR positive samples in EID50/ml

Highest titer in EID50/ml

Number FluDETECT positive (% b)

Mean titer for FluDETECT positive in EID50/ml

H5

5

60/98 (61.2%)

101.60

104.26

17 (28.3%)

102.86

6

53/91 (58.2%)

102.09

104.32

18 (34.0%)

103.46

11

69/109 (63.3%)

101.78

104.00

17 (24.6%)

102.99

H7

5

69/74 (93.2%)

103.13

105.33

40 (58.0%)

103.87

6

78/80 (97.5%)

103.19

105.94

43 (55.1%)

103.92

11

69/73 (94.5%)

102.87

105.55

31 (44.9%)

103.73

  1. aAs a percentage of all samples of the specific virus subtype and pool size
  2. bAs a percentage of samples with CT ≤ 35