Table 3 Intra- and inter-assay reproducibility for TaqMan-based PCR
From: Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay
Concentration of standard plasmid (copies/μl) | Intra-assay variability | Inter-assay variability | ||
|---|---|---|---|---|
\( \overline{X}\pm SD \) | CV (%) | \( \overline{X}\pm SD \) | CV (%) | |
2.97 × 105 | 18.65 ± 0.11 | 0.59 | 18.72 ± 0.12 | 0.66 |
2.97 × 103 | 25.40 ± 0.11 | 0.44 | 25.46 ± 0.16 | 0.63 |
2.97 × 101 | 32.32 ± 0.46 | 1.43 | 32.48 ± 0.72 | 2.21 |